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Área Científica » Trabalhos Premiados » CORRELATION OF THE DEEP OPTIC NERVE HEAD STRUCTURES BETWEEN SDOCT AND CO-LOCALIZED 3D HISTOMORPHOMETRY

Código: TL 012
Apresentação: Oral, segunda opção Pôster
Área Técnica: Glaucoma
Categoria / Classificação: Pesquisa Básica
Região onde foi realizada a pesquisa: Internacional

INSTITUIÇÃO ONDE FOI REALIZADO O TRABALHO:

CONEP:

AUTOR PRINCIPAL:

CO-AUTOR(ES):

TÍTULO:
CORRELATION OF THE DEEP OPTIC NERVE HEAD STRUCTURES BETWEEN SDOCT AND CO-LOCALIZED 3D HISTOMORPHOMETRY

OBJETIVO:
To correlate deep ONH structures within pre-sacrifice SDOCT and post mortem 3-D histomorphometric reconstructions (HMRN).

MÉTODO:
Eighty ONH radial SDOCT B-scans (Heidelberg Spectralis, 870 and 1050 nm – enhanced depth imaging (EDI) were obtained 30 minutes after manometric IOP lowering to 10 mm Hg from both eyes of 4 monkeys with unilateral experimental glaucoma (EG). Each animal was then sacrificed by perfusion fixation, ONH were trephined (6 mm), embedded in paraffin and 3D reconstructed1. SD-OCT B scans were viewed with and without an adaptive compensation algorithm2. Bruch’s Membrane Opening (BMO) was delineated in each SDOCT and 3D HMRN volume3. Each SDOCT data set was 3D co-localized to its 3D HMRN using BMO. Co-localization was qualitatively evaluated using the delineated 3D HMRN vessel tree and the SDOCT infrared image. Each aligned 3D HMRN was digitally re-sectioned to match the associated SDOCT B-scan co-ordinates.

RESULTADOS:
Co-localization of SD-OCT and 3D HMRN volumes was feasible in all 8 eyes. HMRN tissue shrinkage was substantial leading to modest to moderate anatomic distortion. When deep ONH targets including the prelaminar glial columns, anterior laminar insertion, anterior and posterior lamina cribrosa surfaces, anterior neural canal wall and peripapillary sclera were visible within SDOCT B-scans, comparable anatomy was present within the matched 3D HMRN section. EG eye laminar thickening within matched SDOCT B-scans from the control and EG eyes, was confirmed within matched 3D HMRN sections. The compensation algorithm enhanced visualization of peripapillary sclera. Visualization of the lamina was modestly enhanced.

CONCLUSÕES:
Our comparisons suggest anatomic correspondence between SDOCT deep ONH and peripapillary scleral anatomy.

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